The Po River plain is experiencing groundwater resources decline since the last two decades, due to diminished recharge rates and groundwater overexploitation. Additionally, the elevated anthropogenic pressures increase the likelihood of groundwater contamination. For instance, Brescia with more than 200k citizens and a widespread industrial area, hosts many potential sources of groundwater pollution. The managed aquifer recharge (MAR) technique is well-known to mitigate groundwater contamination and to replenish groundwater resources; although MAR could trigger unwanted redox reactions, like pyrite oxidation, that must be evaluated before to establish a MAR scheme. To quantify possible interactions among recharge waters and the aquifer, 2 cores were drilled in a sub-urban area to capture the lithological variability of the unconfined aquifer. To delineate redox gradients within the aquifer, vertical profiles of sediment cores were collected via Rhizon® samplers and analysed for TDS, pH, DOC, major ions, and trace elements. In addition, 3 batches with 160 g of sediment and 800 ml of deionized water were set-up and monitored for 3 months. Pre- and post-experimental characterisation of mineral phases was done via sequential extraction (SE). Stratigraphical cores were similar but not homogeneous, with the most permeable layers constituted by sandy gravel layers. Depth profiles of selected species delineated possible pollution sources from urban leakage, although below admissible limits. SE suggested a >50%±25 increase of Fe-oxides at shallow depths, while no significant increase at the base of the aquifer. The batches and SE demonstrated that no significant release of heavy-metal(loid)s was induced by the recharging water and the already present contaminants were diluted below admissible limits. The proposed framework could be employed to assess the feasibility of MAR to assist tailored design solutions.